If asked, I would say that the first adult was the subject of the observation. Looks wild to me.
All the rearing information is bonus after the initial observation. There is a chance the adult and eggs are not related unless I missed the note about seeing the laying of eggs. They’re probably related though.
“the phenology, and potentially the distribution, would be unrepresentative of the natural biology in cases of culture.”
But your record would be of an ungerminated spore in the place you collected it. So all you are showing is that spores get everywhere. You aren’t saying the fungus would ever have grown in that place, so phenology and habitat are irrelevant. Growing it on in a humid container is just an identification aid.
My main concern with recording the spore would be, was it definitely from the place you claim or was it a subsequent contamination? But I guess mycologists have methods for dealing with that.
I have this observation of E. coli from a creek: https://www.inaturalist.org/observations/38813135
As far as I know the only reliable way to detect the presence of E. coli in water is with a test like this, or it least it’s by far the easiest. It’s an interesting situation to think about since it is indirect evidence in multiple steps.
The coloured dots are a chemical in the gel reacting to the waste produced by the offspring of the original bacteria that were in the creek (each dot represents a colony which was theoretically produced from a single bacterium). My picture would have been taken about 24 hours after the water was collected from the creek. E. coli occurs naturally in the guts of warm-blooded animals, and isn’t supposed to survive longer than 24 hours or so outside of conditions analagous to that (warm, humid, and dark). So the creek isn’t the natural habitat and those individual bacteria would have come from a human (this creek is known to have sewage contamination) or other mammal or bird.
And with all that I still think it should count as wild since I used the date and time of collecting the water sample, and am using it as evidence that the bacteria were occurring naturally in the creek at that time.
Where is the cutoff, though? If I have, say, cyanobacteria and ostracods in an ecosphere, which came from a known locality, but the ecosphere is several months old and might have developed differently during that time than the source waterway… to me that feels “captive” or “cultivated.” Even though, as @tallastro says, I cultured it for identification.
My ecospheres seem like they would be a gray area – I didn’t purposely choose which organisms to put in (they are whatever happened to activate out of the water and sand), but I did build the ecospheres on purpose, knowing stuff would grow in them.
In the case of bacterial culture, as with my ecospheres, there may be succession as the culture grows. In the days it takes for a bacterial culture to become visible, some taxa might have outcompeted and eliminated others.
Okay, so then I can put my ecosphere organisms up as wild, then?
if you look hard enough, you will always be able to find edge cases that are hard to fit into whatever classification system you have. if a tardigrade hitches a ride on a space capsule that stays up in a space for a week, and you observe it before the capsule descends back to earth, what do you record for the location and time? if you observe cells that have been created synthetically, is this life?
here’s what i would try to remember as i classify as wild vs cultivated:
Good to know. I am trying to get at least one observation from every kingdom, and as I believe this to be Genus Oscillatoria, that adds Kingdom Bacteria.
A squirrel I presume planted the acorn. The seedling sprouted under a tree.
We moved the seedling a few yards to a place we want a new tree to grow.
Is the new tree wild or cultivated?
After it was moved yes it is cultivated, we also got a small oak growing randomly and moved it (now it’s a big tree), so when it was growing in initial spot it was wild, but we, humans, decided where it should grow, benefiting it.
Being cheeky here again - what’s wrong with organisms found on the Culture planets/Orbitals? Some may recognise the reference to Iain M. Banks Culture Science Fiction books.